Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels
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چکیده
Methods hMSC cell culture. Human mesenchymal stem cells (hMSCs) were obtained from Lonza Corporation (Wakersville, MD). For encapsulation studies, hMSCs were expanded in growth media (α-MEM, 10% FBS, 1% L-Glutamine & penicillin-streptomycin) and encapsulated at passage 3 in MeHA, MeAlg, MeDex (all at 15 x 10 cells mL) or MeMaHA (10 cells mL) hydrogels. For studies evaluating hMSC differentiation, mixed adipogenic/osteogenic inductive media (“mixed media”) was made by combining commercially available osteogenic and adipogenic inductive media (R&D Systems) in a 1:1 ratio and supplementing with 1% (v/v) penicillin-streptomycin (Gibco). Media changes were performed every 3 days.
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Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels
Although cell-matrix adhesive interactions are known to regulate stem cell differentiation, the underlying mechanisms, in particular for direct three-dimensional encapsulation within hydrogels, are poorly understood. Here, we demonstrate that in covalently crosslinked hyaluronic acid (HA) hydrogels, the differentiation of human mesenchymal stem cells (hMSCs) is directed by the generation of deg...
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